Protein_Domain
RFC105 A
Part:BBa_K1362414:Design
Designed by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha Group: iGEM14_Heidelberg (2014-10-07)
N-terminal start overhang (T)(A)-(G)ATG=RBS+Start RFC[105] A
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is only the sequence of a standard overhang. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[105] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers.
Source
This standard overhang part was developed as part of the iGEM team Heidelberg 2014's Intein Toolbox [1]. All standard sequences can be reviewed in RFC[105] [2].
References
[2] RFC[105]